Figure 1

Hypoxia-inducible accumulation of HIF-1α and HIF-2α in hCMEC/D3 cells depends on confluency state. Cells were exposed to normoxia (21% oxygen) or hypoxia (1% oxygen) for 6 hours. For chemical HIF-1α and HIF-2α induction, normoxic cells were treated with 0.1 mM of the iron chelator deferoxamine mesylate (DFO). HIF-1α and HIF-2α abundance was examined using Western blot analysis.